Table 1 Combination analysis for the cytotoxicity of TQ and GCB against MCF-7 and T47D breast cancer cell lines.

From: Thymoquinone synergizes gemcitabine anti-breast cancer activity via modulating its apoptotic and autophagic activities

Exposure time MCF-7 T47D
24 h IC50 (µM) R-value (%) IC50 (µM) R-value (%)
GCB 32.6 ± 3.6 2.7 ± 0.6 7.6 ± 0.7 6.5 ± 0.5
TQ 80.1 ± 9.8 55.5 ± 1.2 >100 N/A
GCB + TQ 3.4 ± 1.3 0.1 ± 0.03 0.3 ± 0.02 4.3 ± 0.2
CI-value Synergism/0.53 Synergism/less than 0.34
48h
GCB 32.2 ± 1.1 0.5 ± 0.1 6.4 ± 1.4 2.3 ± 0.5
TQ 32.7 ± 1.1 1.2 ± 0.5 104 ± 3.7 N/A
GCB + TQ 3.6 ± 0.7 0.9 ± 0.1 0.27 ± 0.07 1.8 ± 0.4
CI-value Synergism/0.22 Synergism/0.1
72h
GCB 0.9 ± 0.18 0 14.3 ± 2.8 3.2 ± 4.5
TQ 64.9 ± 14.5 1.6 ± 1.3 165.1 ± 2.8 0. 1 ± 0.15
GCB + TQ 0.06 ± 0.01 0 2.3 ± 0.2 0
CI-value Synergism/0.15 Synergism/0.25
  1. Data is presented as mean ± SD; n = 3.