Figure 1 | Scientific Reports

Figure 1

From: Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns

Figure 1

Absorption of bovine milk exosomes in mice. (a) DiR-labeled exosomes (1 × 1012/g body weight) 3, 6 and 24 hours after intravenous injection in whole Balb/c mice (upper panels) and excised tissues (lower panels). (b) Densitometry analysis of fluorescence in excised tissues 3, 6 and 24 hours after intravenous injection of DiR-labeled exosomes. (c) Fluorescence in Balb/c mice 3, 6, 18, 24 or 48 hours after oral gavage of DiR-labeled (right mouse) or unlabeled exosomes (left mouse; 1 × 1012/g body weight). (d) Fluorescence in excised organs of Balb/c mice 3, 6, 18, 24 or 48 hours after oral gavage of free DiR, unlabeled or DiR-labeled exosomes (1 × 1012/g body weight). (e) Densitometry analysis of fluorescence in excised tissues after oral gavage of unlabeled or DiR-labeled exosomes at 24 hours, normalized for plate background for each mouse analyzed. (f) Dose-response analysis of fluorescence in excised murine tissues 24 h after intravenous injection with unlabeled or DiR-labeled exosomes (1 × 1010/g, 1 × 1011/g, 1 × 1012/g body weight). (g) Densitometry analysis of excised tissues after intravenous injection of unlabeled or DiR-labeled exosomes for each mouse analyzed (1 × 1010/g, 1 × 1011/g, 1 × 1012/g body weight). (h) Dose-response analysis of fluorescence in excised murine tissues 24 h after oral gavage with unlabeled or DiR-labeled exosomes (1 × 1011/g, 1 × 1012/g body weight) 24 h after oral gavage. (i) Densitometry analysis of excised tissues after oral gavage of unlabeled or DiR-labeled exosomes in dose-response experiments. Panels in this figure were assembled from multiple independent images and gels; individual images in the grouped figure are separated by white space.

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