Tissue parasite distribution in mice chronically infected with T. cruzi after oral transmission. (a) Representative ex vivo images of tissue-specific parasite bioluminescence at 4 months post-infection. Images show infection distributions and intensities for adipose, gut mesenteric tissue, heart, lung, large intestine, liver, skeletal muscle, spleen, small intestine and stomach. Log-scale pseudocolour heat-maps show intensity of bioluminescence; minimum and maximum radiances are indicated. Tissues and organs are arranged as shown (bottom right). (b) Charts show the proportion of animals that had above threshold bioluminescence in the indicated organs and tissue samples. The category “Other” refers to bioluminescence signals in the animal carcass that could not be unambiguously assigned to a specific organ. MT oral (intra-gastric) n = 6, MT i.p. n = 12, BT oral (buccal) n = 4, BT i.p. n = 12.