Ex vivo tissue bioluminescence imaging of mice acutely infected with T. cruzi after oral transmission. Mice were infected with 105 metacyclic or bloodstream T. cruzi trypomastigotes (MT, BT) by i.p. injection, oral gavage [p.o. (g)] into the stomach or deposition in the oral cavity [p.o. (b)]. Parasite distribution was assessed by bioluminescence signal in organs and tissue samples from mice at 6 days post-infection. Log-scale pseudocolour heat-maps show intensity of bioluminescence; minimum and maximum radiances are indicated. Samples were arranged as indicated in the table. Images are representative of three independent replicates with the table showing the overall assessment of T. cruzi presence vs. absence in each sample.