The working principle of a rapid detection in the integrated detection system ISAAC by accelerating the assay with a PDMS concentrator integrated into each well. (a) Schematic of a standard microplate-based ISAAC with PDMS concentrator chips inserted into the wells and reversibly bonded onto the bottom surfaces. (b) A reaction mixture containing an analyte (e.g., MMP-9) is loaded into the concentrator chip. (c) A molecular concentration plug containing the reaction mixture as well as the reaction product forms on the anodic side of the PEDOT:PSS membrane as a result of the balance between the ion depletion zone and electroosmosis when voltage is applied across the microchannel. The dotted area indicates the computational domain for numerical simulation of ICP.