MiR-494-3p promotes PI3K/AKT pathway hyperactivation and human hepatocellular carcinoma progression by targeting PTEN

Recent studies have shown that miR-494-3p is oncogene and has a central role in many solid tumors; however, the role of miR-494-3p in the progression and prognosis of hepatocellular carcinoma (HCC) remains unknown. In this study, it was found that miR-494-3p was up-regulated in HCC tissues. The high level of miR-494-3p in HCC tumors was correlated with aggressive clinicopathological characteristics and predicted poor prognosis in HCC patients. Functional study demonstrated that miR-494-3p significantly promoted HCC cell metastasis in vitro and vivo. Since phosphoinositide 3-kinase/protein kinase-B (PI3K/AKT) signaling is a basic oncogenic driver in HCC, a potential role of miR-494-3p was explored as well as its target genes in PI3K/AKT activation. Of all the predicted target genes of miR-494-3p, the tumor-suppressor phosphatase and tensin homolog (PTEN) were identified. In conclusion, the data we collected could define an original mechanism of PI3K/AKT hyperactivation and sketch the regulatory role of miR-494-3p in suppressing the expression of PTEN. Therefore, targeting miR-494-3p could provide an effective therapeutic method for the treatment of the disease.

progression and metastasis by post-transcriptional regulation to CXCR4 mRNA 18 . However, function and characterization of miR-494-3p in patients with HCC has not been investigated and remains unclear [19][20][21] .
This study investigated the relationship between levels of miR-494-3p in HCC patient specimens and its outcome and revealed that miR-494-3p could promote the metastasis of HCC in vitro and in vivo. We also found the molecular mechanism that possibly underlies the functions of miR-494-3p in HCC, which might be that PTEN is a new target gene of miR-494-3p. Taken together, these data indicated that miR-494-3p could be a possible biomarker for diagnosing HCC as well as a target for developing novel therapies for the treatment of HCC.
Results miR-494-3p is up-regulated in HCC tissues and associated with aggressive clinicopathological features. In order to investigate the expression and clinical significance of miR-494-3p in HCC, the expression of miR-494-3p was detected in 271 paired primary HCC tissues and corresponding adjacent non-tumor samples. qRT-PCR indicated that the average expression level of miR-494-3p was significantly higher in the cancerous tissues than in the adjacent non-cancerous tissues (Fig. 1A, p < 0.001). Consistently, miR-494-3p was up-regulated in 68.3% (185/271) of the tested HCC tissues compared to that in matched non-cancerous counterparts (Fig. 1B). Moreover, miR-494-3p expression was increased in HCC patients with larger tumors (>5 cm), multiple tumor number, advanced-stage (stage III-IV) and early recurrence (Fig. 1C-F). To further explore the relations between miR-494-3p expression levels and its clinicopathologic characteristics, we divided the 271 HCC patients into two subgroups, the high and low miR-494-3p expression subgroups, based on the median miR-494-3p expression. As shown in Table 1, high miR-494-3p expression group was correlated with lager tumor size (p = 0.028), larger tumor sizes (≥5 cm) (p = 0.032), multiple tumors (n ≥ 2) (p = 0.041) and advanced TNM stages (p < 0.001). It was indicated by the univariate analysis that of all the clinicopathological characteristics, the miR-494-3p expression level, tumor size, tumor number, vascular invasion, TNM stage, and BCLC stage were correlated with RFS, and the miR-494-3p expression level, tumor size, tumor number, AFP level, vascular invasion, TNM stage, and BCLC stage were correlated with OS (Supplementary Table 1). Moreover, multivariate analysis indicated that miR-494-3p expression levels, along with TNM stage, tumor size and tumor number, are independent risk factors for both recurrence-free survival (RFS) and overall survival (OS) in HCC patients (Table 2). Collectively, these data indicated that increased miR-494-3p expression could be correlated with malignant progression in HCC patients. miR-494-3p upregulation predicted poor prognosis in HCC patients. We further analyzed the association between the miR-494-3p expression and the prognosis of HCC patients after hepatectomy. It was found that the miR-494-3p high-expression group showed significantly poorer RFS (P = 0.003, Fig. 2A) and poorer OS (P < 0.001, Fig. 2B). A Subgroup analysis showed that among patients with AFP negative (146 patients), the difference in RFS and OS between the miR-197-3p high and low-expression groups still existed (P = 0.0012, P = 0.0002; Figure 1. miR-494-3p is up-regulated in HCC tissues and associated with aggressive clinicopathological features. (A and B) miR-494-3p expression levels were compared between HCC tissue samples and paired adjacent non-tumor tissue samples. U6 was used as an internal control to normalize the expression level of miR-494-3p. (C) miR-494-3pexpression levels were examined in HCC tissues with and without vascular invasion. (D) miR-494-3p expression levels were examined in larger and smaller HCC tissue samples. (E) miR-494-3p levels were compared between TNM III-IV HCC tissues and TNM I-II HCC tissues. (F) miR-494-3p levels were compared between HCC tissues exhibiting late recurrence and HCC tissues exhibiting early recurrence. (*p < 0.05, **p < 0.01, ***p < 0.001).
SciEnTiFic RepoRts | (2018) 8:10461 | DOI:10.1038/s41598-018-28519-2 respectively, Fig. 2C,D). Further analysis indicated that of the patients who were tumor size <5 cm (121 patients), the miR-197-3p high-expression group tended to correlate with poor RFS but without statistical significance had poorer (P = 0.0788, Fig. 2E) and poor OS (P = 0.0436; Fig. 2F). As a whole, the data above indicated that the expression level of miR-197-3p could be adopted as an independent factor for predicting the prognosis of HCC.

miR-494-3p promoted metastasis and invasion of HCC cell in vitro and in vivo.
To explore the basic role of miR-494-3p in HCC progression, firstly we examined the levels of miR-494-3p in several human HCC cell lines (HCCLM3, Hep3B, HepG2, Huh7, and SMMC7721) and normal liver cells (THLE-3). qRT-PCR   revealed that miR-494-3p level was markedly increased in all five HCC cell lines compared to that in THLE-3 cell line ( Supplementary Fig. 1A). SMMC7721cells and HCCLM3cells were selected for gain-and loss-of-function study. The transfection efficiency was validated by qRT-PCR (p < 0.001, Supplementary  . In additional, CCK-8 assay and qRT-PCR indicated that miR-494-3p did not influence HCC cell growth and apoptosis ( Supplementary Fig. 3). To verify the function of miR-494-3p in vivo, SMMC-NC and SMMC-Inhibitor cells were injected directly into the tail veins of nude mice in order to establish a animal model of lung metastasis. As SMMC-NC and SMMC-Inhibitor express firefly luciferase, the process of lung metastasis for 0 and 70 days was monitored dynamically through an in vivo imaging system. The results of the photon flux revealed that miR-494-3p down-expression inhibited lung metastasis (Fig. 3E,F). After 70 days, the lungs were dissected and stained with H&E. It was found that the lungs in the control group displayed markedly more micrometastases, compared with those in the other group (Fig. 3G,H). Collectively, these findings indicated that miR-494-3p could be essential for HCC cell invasive and metastatic potential.

miR-494-3p represses PTEN expression and activates PI3K in hepatocellular carcinoma cells.
To clarify the molecular mechanism underlying the functional effects of miR-494-3p in HCC progression, we looked for candidate target genes of miR-494-3p using in public databases, including TargetScan (http://www.targetscan.org/) and miRanda (microrna.org and miRBase). It was found that the 3′UTR of the tumor-suppressor PTEN mRNA contained the complementary sequence of miR-494-3p (Fig. 4A). To confirm whether miR-494-3p directly targets the 3′UTR of PTEN, we cloned a fragment of the 3′UTR of PTEN mRNA that harbored the predicted binding site of miR-494-3p and inserted it into a luciferase reporter plasmid.
Overexpression miR-494-3p significantly suppressed luciferase activity from the wild-type reporter but not from the mutant reporter, which indicated that the 3′-UTR of PTEN could be targeted by miR-494-3p and that the point mutations in this sequence might abolish this effect in SMMC7721 and HCCLM3 (Fig. 4B,C). Besides, the protein levels of PTEN were significantly reduced after overexpression miR-494-3p, while the protein level of PTEN was significantly increased in miR-494-3p knockdown in SMMC-7721 and HCCLM3 cells (Fig. 4D). In contrast, we observed no significant changes for PTEN mRNA levels ( Supplementary Fig. 4). These results indicated that miR-494-3p could suppress PTEN protein expression through translational repression.
To determine if the PTEN gene is required for the miR-494-3p's effects on HCC cell metastasis, ectopic over-expression of PTEN was performed to conduct functional studies in SMMC7721 and HCCLM3 cells. In Fig. 4E,F, the capacities of migration and invasion in PTEN-overexpression HCC cells were significantly inhibited, while overexpression of PTEN abolished the effects of miR-494-3p on of HCC cells migration and invasion ability. Therefore, overexpressed PTEN abolished the effects of miR-494-3p on phenotypes of HCC cells. By counteracting AKT activation, PTEN serves as a key negative regulator of PI3K signaling. The statuses of the pathway in SMMC-NC and SMMC-Mimic cells were analyzed by measuring the phosphorylation (activation) of AKT. The results revealed that phospho(p) AKT level in SMMC-Mimic cells was markedly higher than that in SMMC-NC cells. Moreover, the level of activated AKT was significantly lowered through the transfection of SMMC-Mimic cells with green fluorescent protein (GFP)-PTEN but not with the empty vector ( Supplementary  Fig. 5). Taken together, these results could provide a proof of concept for the ability of miR-494-3p to modulate PI3K signaling by silencing PTEN.
MiR-494-3p was recognized as an oncogene in lung cancer 31 . Nevertheless, the characterization of miR-494-3p in HCC and its association with cancer progression and development remain unknown. In our study, we found that miR-494-3p was up-regulated in HCC tumor tissues and HCC cell lines. miR-494-3p up-regulation was found to correlated with high, larger tumor sizes (≥5 cm), multiple tumors (n ≥ 2) and TNM stage. The correlation between TNM stage and the expression of miR-494-3p indicates that miR-494-3p may be used as evaluating malignant degree. Furtherly, its correlation with tumor size, tumor number and vascular invasion showed that miR-494-3p played a role in hepatocarcinogenesis.
A subset of patients with tumor size ≤5 cm, who had been predicted to have better outcomes by adopting the standard staging system 32-34 , showed poor prognosis instead, which suggested that a complementary prognostic predictor was needed for the patients. Further prognostic analyses showed that tumor size ≤5 cm with higher miR-494-3p expression also had poorer OS (p = 0.0436). These results indicated that miR-494-3p measurement maybe help clinicians identify the early-stage patients with high recurrence risk and recommend appropriate follow-up and adjuvant therapies for these patients.
Serum AFP, now the most widely-used biomarkers for the diagnosis and treatment of HCC [35][36][37] , is also used to predict the possibility of recurrence for AFP-positive HCC patients after hepatectomy 38,39 . However, there still lacks a quick, simple and effective marker to monitor the recurrence of the disease and to guide treatments for AFP-negative HCC patients, who account for 30-40% of all HCC patients [40][41][42][43] . It was found that in AFP-negative patients, high miR-494-3p expression had significant relationship with poor RFS. MiR-494-3p could also be a potential biomarker for predicting the recurrence risk for AFP-negative HCC patients.
The significant association between miR-494-3p expression in tumors along with the aggressive clinical behaviors and poor prognosis of HCC patients urged us to explore whether miR-494-3p plays a functional role in HCC progression and dissemination. It turned out that both the in vitro and in vivo data demonstrated that miR-494-3p inhibited hampered the invasion and metastasis of HCC cells; while overexpression miR-494-3p enhanced invasion and metastasis ability of HCC cells. The results also suggested that miR-494-3p could serve as a promising target for therapeutic intervention against invasive and metastatic HCC. Previous reports have shown that the activation of PI3K/AKT contributes to cell growth, promotes invasion and EMT [44][45][46] . By using TargetScan bioinformatics, this study identified the PTEN gene as a possible direct target for miR-494-3p. Through performing a luciferase reporter assay, real-time PCR and Western blotting, our results verified that PTEN inactivation by miR-494-3p shed light on the mechanism and positive feedback circuits that mediate the activation of the PI3K pathway in HCC carcinogenesis. The role of miR-494-3p/ PI3K/AKT axis in HCC progress might expand the key functional pathways to abnormal invasion of HCC cells.
In summary, we found that miR-494-3p expression was frequently increased in HCC tumor tissues and may serve as a prognostic bio-marker in patients with HCC. Mechanically, our results indicated that miR-494-3p promoted HCC cell metastasis by directly suppressing the expression of PTEN, which not only sheds new light on HCC progression and metastasis, but also provides a potential target for cancer prevention and treatment.

Materials and Methods
Ethics statement. All  Complementary DNA (cDNA) was amplified and quantified on CFX96 system (BIO-RAD, USA) using iQ SYBR Green (BIO-RAD, USA). U6 or β-actin was adopted as endogenous controls. Relative fold expressions were calculated by the comparative threshold cycle (2-ddCt) method. Mature miR-494-3p expression was detected using a TaqMan miRNA-assay kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions. RNU6B gene was used as a normalization control. All experiments were performed in triplicate and repeated once. The β-actin gene was used as an internal control. PCR was run using the following conditions: 30 cycles consisting of denaturation at 94 °C for 30 sec, annealing at 56 °C (58 °C for β-actin) for 30 sec, and extension at 72 °C for 30 sec. Each PCR product was separated using 1.5% agarose gel electrophoresis and visualized using ethidium bromide staining. The primer and probe sequences used in the qRT-PCR reactions are listed in Supplementary Table 2.
Western blotting analysis. Cell protein lysates were separated using 10% sodium dodecyl sulfate polyacrylamide gels, electrophoretically transferred to polyvinylidene difluoride membranes (Roche Diagnostics, Mannheim, Germany), and then detected using PTEN antibodies (ab107918), pAKT (sc-7985-R). Protein loading was measured using a mouse anti-GAPDH monoclonal antibody (mAbcam 8245). Lab Works Image Acquisition and Analysis Software (UVP, Upland, CA, USA) were adopted to quantify the band intensities. Luciferase activity assay. The 3′UTR of PTEN was amplified and cloned downstream of the pGL3/ Luciferase(Luc)vector. The mutant 3′UTR of PTEN was amplified using the pGL3/Luc-PTEN 3′UTR as the template and then was cloned downstream of the pGL3/Luc vector. For the luciferase reporter assay, cells were co-transfected with either miR-494-3p Inhibitor-s or control and the pGL3/Luc-PTEN 3′UTR or the mutant 3′UTR, together with the controls. At 48 h after transfection, the cells were lysed using RIPA buffer, and luciferase intensity was measured using an F-4500 Fluorescence Spectrophotometer (HIT-ACHI).
Cell proliferation (MTT) assay and colony formation assay. The transfected cells were plated into 96-well plates at a density of 5,000 cells/well. At 48 h after transfection, the cells were incubated with MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) for 4 h at 37 °C. The cells were then agitated with MTT solvent on an orbital shaker for 10 min while avoiding light. The absorbance was measured at 450 nm (OD450nm) using a spectrophotometer.
Animal studies. To investigate the effects of miR-494-3p on HCC metastasis in vivo, the lateral tail vein injection model was adopted to assess the potential of the tumor cells to metastasize to the lungs. The metastases of the lungs were monitored through an IVIS@ Lumina II system (CaliperLife Sciences, Hopkinton, MA, USA) for 10 min after intraperitoneal injection of 4.0 mg of luciferin (Gold Biotech) in 50 µl of saline. The nude mice were housed in cages under standard conditions, and the experiments were performed in accordance with the requirements of the Second Military Medical University Animal Care Facility and the National Institutes of Health guidelines. The mice were maintained in pathogen-free conditions. Statistical analysis. All values are presented as means ± standard deviation (SD). Significant differences were determined by GraphPad 5.0 software (USA). Student's t-test was adopted to determine statistical differences between two groups. One-way ANOVA was adopted to determine statistical differences between multiple tests. The chi-square test was used to analyze the relationship between miR-494-3p expression and clinicopathological characteristics. Survival curves were plotted by the Kaplan Meier method and compared by log-rank test. P < 0.05 was considered significant. All the experiments were repeated three times.