Repeated liraglutide effect on glucose intolerant 5-month-old Wfs1 KO rats. (a) IPGTT (2 g/kg) before and after 8-day chronic administration of 0.4 mg/kg liraglutide to 5-month-old male Wfs1 KO and control rats. (b) Decrease in IPGTT (2 g/kg) area under curve before and after chronic liraglutide treatment for 8 days. Glucose-stimulated increases in blood (c) insulin and (d) C-peptide levels before and 30 minutes after glucose administration. The data were compared using repeated measures ANOVA followed by Tukey’s HSD tests; £££p < 0.001 compared to WT AUC during same IPGTT. ***p < 0.001 compared to baseline of the same genotype during the same IPGTT. ##p < 0.01 compared to WT animals in the same timepoint during the same IPGTT. ¤p < 0.05 compared to WT saline animals in the same timepoint during the first IPGTT. The data are presented as the mean ± SEM, n = 6–8.