Retinal-like molecules exhibited no effect on PIP2 upon blue light exposure. Images of HeLa cells expressing PIP2 sensor (mCherry-PH) on PM. In all of the experiments conducted, cells were exposed to blue light (4.86 µW of 445 nm) which is indicated by the white box. Cells were incubated with (A) β-ionone (50 µM), (B) 10E, 12Z linoleic acid (50 µM), (C) Retinol (50 µM) and (D) Retinoic acid (50 µM), for 10 minutes followed by continuous exposure of short pulses of blue light for 200 s. In all experiments, cells did not show a detectable PIP2 sensor translocation. Plots show the dynamics of PIP2 sensor translocation in cells shown in A–E. (mean ± S.E.M., n = 5–10 cells). Mean and S.E.M. are from 3 < independent experiments. (blue light (BL) = blue box). Scale = 5 µm.