Figure 4 | Scientific Reports

Figure 4

From: Rapid recombinant protein expression in cell-free extracts from human blood

Figure 4

(A) Expression of luciferase using leukocyte cell-free system. (B) Quantification of G-CSF produced (expression time −2 h) using leukocyte cell-free system. G-CSF standard curve generated using a quantitative Sandwich ELISA (G-CSF Human ELISA, Abcam, USA) following the manufacturer’s instructions. All materials required for the analysis was provided in the kit. For the standard curve, a dilution series containing 0 to 500 pg/mL of G-CSF standard was prepared. (C) Quantification of EPO (expression time − 2 h) produced using leukocyte cell-free system. For reference standard protein concentrations, a dilution series containing 0 to 100 mIU/mL of EPO standard was prepared. The concentration of EPO in the harvest sample was determined using a quantitative Human EPO ELISA Kit, Abcam, USA) following the manufacturer’s instructions. All materials required for the analysis were provided in the kit. Blank represents no-DNA control. The error bars represent 90% confidence intervals (n = 3).

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