Transmission electron microscopy of cataract solutions treated with 25-hydroxycholesterol, doxycycline, and rosmarinic acid. TEM micrographs of cataract particles in BSS in the absence (A,E,I) or presence of increasing concentrations (50, 500 μM, and 1 mM) of 25-hydroxycholesterol (B–D) doxycycline (F–H), or rosmarinic acid (J–L). Reaction mixtures were incubated for 2 days as described, negatively stained and imaged. Scale bars, 2 µm. Representative electron micrographs from three different experiments conducted with each compound are shown.