Ex vivo assay for screening of human cataract modulating compounds. (A) Schematic outline of the screening system. Human cataract lens fragments were removed from patients undergoing routine cataract surgery. Solutions containing the cataract material were then exposed to modulating compounds and the results were examined. (B) Non-biased screening of known amyloid modulators for a cataract modulating effect was performed. Colors correspond to the effect on turbidity of cataract solutions: dark gray: active; medium gray: partially active; light gray: inactive. The disaggregation of human cataract samples was monitored by absorbance measurement at λ = 340 nm and 37 °C. Columns denoted by * indicate statistical significance of the results.