Figure 2 | Scientific Reports

Figure 2

From: Kcnn4 is a modifier gene of intestinal cystic fibrosis preventing lethality in the Cftr-F508del mouse

Figure 2

Effect of inactivation of Cftr and Kcnn4 on electrophysiology and histology of intestinal epithelium. (AD) Representative Vte traces of the four genotypes are presented. Number 1 indicates addition of 10 µM amiloride to the apical side. Number 2 indicates the addition of cAMP-increasing cocktail (100 µM IBMX+ 1 µM forskolin) to induce cAMP-activated anion secretion that is seen as a negative negative deflection in Vte. cAMP-activated basolateral potassium channel KCNQ1/KCNE3 is inhibited with serosal 10 µM chromanol 293B (Number 3). Finally Ca2+-activated anion secretion is elicited by serosal addition of 100 µM carbachol (Number 4). All drugs are maintained constant after their corresponding addition to the bath solution. (E) Summary of calculated changes in short-circuit current under the different treatments. Values are given as means ± S.E.M., n = 3 for each group; *indicates p < 0.002 (unpaired Student’s t test) for comparisons with wild type (WT). (F–I) PAS staining of ileum histological sections showing mucus accumulation in the CF animals. Representative images of 5–6 samples for each group. (J) Summary of faecal water content in mice of the different genotypes. Values are given as means ± S.E.M., n = 5–7; *indicates p < 0.003 and n.s. is no statistical difference, unpaired Student’s t test.