Milk cholesterol concentration in mice is not affected by high cholesterol diet- or genetically-induced hypercholesterolaemia

Breast milk cholesterol content may imply to affect short- and long-term cholesterol homeostasis in the offspring. However, mechanisms of regulating milk cholesterol concentration are only partly understood. We used different mouse models to assess the impact of high cholesterol diet (HC)- or genetically-induced hypercholesterolaemia on milk cholesterol content. At day 14 postpartum we determined milk, plasma and tissue lipids in wild type (WT), LDL receptor knockout (Ldlr−/−), and ATP-binding cassette transporter G8 knockout (Abcg8−/−) mice fed either low- or 0.5% HC diet. In chow-fed mice, plasma cholesterol was higher in Ldlr−/− dams compared to WT. HC-feeding increased plasma cholesterol in all three models compared to chow diet. Despite the up to 5-fold change in plasma cholesterol concentration, the genetic and dietary conditions did not affect milk cholesterol levels. To detect possible compensatory changes, we quantified de novo cholesterol synthesis in mammary gland and liver, which was strongly reduced in the various hypercholesterolaemic conditions. Together, these data suggest that milk cholesterol concentration in mice is not affected by conditions of maternal hypercholesterolaemia and is maintained at stable levels via ABCG8- and LDLR-independent mechanisms. The robustness of milk cholesterol levels might indicate an important physiological function of cholesterol supply to the offspring.

B-containing lipoproteins are abundantly expressed in the mammary epithelial cells, amongst which LDL-, VLDL-and CD36-receptors 14 . Other lipoproteins found in plasma, like the high-density lipoproteins, may serve as an alternative source for cholesterol uptake since scavenger receptors from the CD36 family are also expressed in the mammary epithelium 16 . In addition, mammary gland epithelial cells express cholesterol efflux transporters, such as ATP-binding cassette (ABC) transporters ABCG5/ABCG8, ABCA1, and ABCG1, whose expressions fluctuate depending on lactation stage [17][18][19] and could possibly impact cholesterol levels in the milk.
We aimed to address the relationship between maternal hypercholesterolaemia and milk cholesterol concentration in mouse models. We analysed milk cholesterol concentrations in lactating mice with hypercholesterolaemia of different severity, induced by dietary and/or genetic manipulations. The dietary means to manipulate plasma cholesterol concentrations consisted of feeding a high-cholesterol diet (0.5% w/w), while genetic manipulation involved the ablation of either the Abcg8 or the Ldlr gene. The ABC cassette G8 protein is a cholesterol transporter primarily expressed on the apical membrane of hepatocytes and enterocytes, where it facilitates export of cholesterol 20 . Interestingly, Abcg8 is also moderately expressed in the lactating bovine mammary gland and in the murine mammary gland, as demonstrated in literature and online databases 17,19,21 . The LDL-receptor is the dominant transport protein involved in the uptake of apoB100-containing lipoproteins from the plasma 22 , and highly expressed in murine mammary gland 21 . Humans with genetic loss of LDLR function have a severe hypercholesterolaemia that is further increased upon dietary cholesterol exposure 23 . We assessed the potential relevance of cholesterol secretion into milk via the ABCG8 transporter and via mammary gland uptake of cholesterol via the LDL receptor. To assess possible variation in the origin of milk cholesterol in the different models of hypercholesterolaemia, we measured de novo cholesterol synthesis in the liver and mammary gland, using deuterated water methodology.

High-cholesterol diet increases plasma and hepatic cholesterol levels.
To assess the isolated effect of ABCG8-or LDLR-deficiency we first measured cholesterol levels in plasma of dams on a chow diet. While ABCG8-deficiency did not affect basal plasma cholesterol, the LDLR-deficient dams displayed marked hypercholesterolaemia (5.2-fold change, p < 0.01, Fig. 1a), mostly due to increased cholesterol levels in LDL and VLDL ( Fig. 1b-d). Feeding the dams high cholesterol (HC) diet increased the levels of total plasma cholesterol in all models (Fig. 1a). The size of the effect reached maximum in the Ldlr−/− mice (4.8-fold change, p < 0.01) followed by Abcg8−/− (2-fold change, p < 0.05) and wild-type (1.5-fold change, p < 0.05). On chow diet, hepatic cholesterol concentration corresponded with the differences in the plasma cholesterol levels: similar levels in wild-type and Abcg8 knockout mice and 0.6-fold higher in LDLR-deficient mice (p < 0.01). The HC diet increased the cholesterol accumulation in the hepatic tissues of all dams (p < 0.05, Fig. 2). On the HC diet, however, the hepatic cholesterol concentrations did not differ significantly between the three models.
Milk cholesterol levels are independent of plasma, liver and mammary gland cholesterol levels. We then determined whether the hypercholesterolaemia was associated with increased cholesterol content of the mammary glands. On chow diet there were no differences in mammary cholesterol content between genotypes, despite the significantly increased plasma cholesterol levels in Ldlr−/− mice (Fig. 3a). The HC diet did not increase mammary cholesterol content in the WT mice, in contrast to the Abcg8 and Ldlr knockout mice (+39%, p < 0.05; and +62%, p < 0.01 respectively; Fig. 3a). Interestingly, the HC diet-induced hypercholesterolaemia did not affect the milk cholesterol concentrations in any of the three models, with milk cholesterol levels ranging between 1.7-2.3 mM (interquartile range) (Fig. 3b).
In order to analyse the possible association between milk cholesterol levels and plasma and mammary gland cholesterol levels and nest size, we performed regression analysis. Nest sizes (range: 2-8 pups) were not correlated with milk cholesterol levels. Cholesterol levels in mammary gland tissue were strongly and positively related to plasma cholesterol levels in all three models (WT r 2 = 0.54, p = 0.016; Abcg8−/− r 2 = 0.61, p = 0.013; Ldlr−/− r 2 = 0.51, p = 0.0096). In none of the three groups were plasma and milk cholesterol levels significantly correlated. Ratios of VLDL + LDL to HDL cholesterol as calculated from FPLC fractions (Fig. 1e) were also unrelated to milk cholesterol levels.
De novo cholesterol synthesis is strongly decreased in high cholesterol-fed mice. The increased plasma, hepatic and mammary gland cholesterol levels in the hypercholesterolaemic models did not translate into increased milk cholesterol concentrations. We then tested the possibility that the stable concentrations were obtained by suppression of systemic or local cholesterol synthesis. In all chow-fed groups there was de novo hepatic and mammary gland cholesterol synthesis (Fig. 4). Feeding the HC diet strongly reduced the cholesterol synthesis rate in liver (Fig. 4a) and mammary gland (Fig. 4b) in all three models.
Next, we used linear regression analysis to assess the possible relationship between the de novo synthesis in mammary gland and the milk cholesterol concentration. The milk cholesterol levels did not correlate with the fraction of de novo synthesized cholesterol in mammary gland in any of the three groups (WT r 2 = 0.03, p = 0.66; Abcg8−/− r 2 = 0.07, p = 0.51; Ldlr−/− r 2 = 0.05, p = 0.64).

Discussion
We addressed the relationship between maternal hypercholesterolaemia, induced by dietary or genetic means, and milk cholesterol concentrations in mice. Our data demonstrate that milk cholesterol concentration is not affected by induction of severe hypercholesterolaemia and increased cholesterol levels in liver and mammary gland. Clearly, the ABC-cassette transporter ABCG8 and the LDL receptor do not have a critical role in defining milk cholesterol concentration, since their inactivation did not change it. Our data demonstrate the apparent robustness of milk cholesterol levels, which could support important physiological functions for the offspring. The milk cholesterol concentration was not affected by genetic inactivation of two candidate genes with a possible role in cholesterol transport towards milk, nor by high cholesterol diet-induced hypercholesterolaemia. This observation indicates that either the gene products are not involved, or that alternative transporting mechanisms ensure redundancy in the supply of cholesterol destined for secretion into the milk. The hypothesis that the LDL receptor is involved in milk cholesterol transport was based on findings describing an association between lactation and increased mammary gland expression of LDLR in human subjects 14 and high LDLR expression in the murine mammary gland 21 . In addition, lactation in rodents is characterized by an increase in circulating LDL 24 , compatible with a role for the low-density lipoproteins as a source for milk cholesterol. Our data indicate that uptake of cholesterol by the mammary gland can be conducted quantitatively by LDLR-independent mechanism(s). We cannot exclude that an alternative, LDLR-independent mechanism involves an alternative receptor for LDL uptake. In support of this notion, radioactivity studies in mice have shown the transfer of ApoB100 across the mammary epithelium towards the milk to take place at the same extent in both wild-type and LDLR-deficient mice 15 . Possibly VLDL and LRP receptors 25 , or even CD36 26 can substitute for LDLR-deficiency. The hypothesis that ABCG8 is involved in milk cholesterol transport rests on the increased expression levels of the heterodimer ABCG5/ABCG8 in lactating bovine mammary glands 17,19 , and appreciable expression of ABCG8 in murine mammary gland 21 . In hepatocytes and intestinal epithelial cells the ABCG5/ABCG8 dimer is expressed at the apical membrane 20 where it is essential for the export of free cholesterol towards the bile and intestinal lumen, respectively 27 . Our data, however, does not support a critically important role for ABCG8 in the process of cholesterol efflux across the mammary gland epithelium. The unchanged plasma cholesterol levels in Abcg8−/− mice on chow may be related to the fact that the diet used contained no cholesterol. Apparently neither the LDL receptor nor ABCG8 is crucial for cholesterol transport towards milk in our experimental setup. In order to further explore the mechanistic effects of genetic ablation of LDLR and ABCG8 on milk production, additional studies in an in vitro model would be helpful. Unfortunately, however, there is no established reliable in vitro system for lactating mammary gland cells available to study alveolar mammary gland epithelial cell cholesterol transfer 28 .
De novo cholesterol synthesis has been shown to contribute to milk cholesterol 12 . For the dams, cholesterol demand is increased during lactation, corresponding with increased expression of cholesterol synthesis genes in both liver and mammary glands of bovines, rodents and humans 13,14,29 . We found 12-fold higher fractional cholesterol synthesis rates in liver compared to mammary gland, which is in agreement with previous studies demonstrating a larger contribution to milk cholesterol originating from hepatic than from mammary synthesis 12 . The lower mammary gland cholesterol synthesis compared with hepatic synthesis also corresponds to the expression levels of the Hmgcr gene in the two tissues, encoding for the rate-limiting enzyme of cholesterol synthesis 13 . In each of the three murine genotypes, dietary cholesterol supplementation strongly decreased de novo cholesterol synthesis in liver and in mammary gland, similarly to observations in rats 30 . The decreased de novo synthesis rates in liver and mammary gland, however, did not decrease milk cholesterol levels. The cholesterol synthesis rate is apparently not a critical driver for the amount of cholesterol secreted into milk. Rather, it seems that milk cholesterol concentration is robust and "protected" against profound hypercholesterolaemia despite strongly increased tissue cholesterol levels. In addition, comparable to respective compensatory changes in other organs such as the liver, also in the mammary gland cholesterol synthesis decreased in response to dietary cholesterol feeding.
The use of whole-body inactivation of specific genes, as utilized in this study, is comprehensively associated with systemic changes in cholesterol metabolism and apolipoprotein balance. Employing mammary gland-specific genetic models would exclude the influence of hepatic or intestinal deficiency in our mice. However, the present lack of influence on milk cholesterol concentration in whole body-knockouts does not support the possibility that organ-specific inactivation would greatly affect milk cholesterol concentrations.
We would like to hypothesize on the physiological explanation(s) of the present findings. First, it is tempting to speculate that the apparent robustness of the cholesterol concentration in milk relates to physiological importance in milk secretion. The importance of a stable milk cholesterol concentration could relate to the process of secretion of milk lipids, in particular triglycerides. Within the alveolar cells of the mammary gland the secretory lipids are shaped in single phospholipid layer-wrapped lipid droplets. During exocytosis the lipid droplets acquire an additional cholesterol-rich phospholipid bilayer, resulting in the formation of the milk-fat globule (MFG) 31 . Milk cholesterol is mainly present as unesterified cholesterol in the MFG-membrane (85-90%) and the other part as cholesteryl esters in the MFG-core 32,33 . The packaging of the lipid droplets with the MFG membrane, which is essential for their secretion, may therefore translate into a rather stable cholesterol content in milk, based on its role as an emulsion-stabilizing component as part of the MFG-membrane. Second, the robust cholesterol concentration in milk could also underline the hypothesized physiological function of milk cholesterol for later health of the offspring. In contrast to breast milk, the fat globules of common infant milk formula are smaller in size and differ in composition, being coated with milk proteins instead of a phospholipid and cholesterol-rich  34,35 . Indeed, infant formulas hardly contain cholesterol 3 . Cholesterol in early life is not considered an essential dietary component since infants are capable of de novo cholesterol synthesis, and thus do not critically depend on milk for their cholesterol supply. As expected, infants fed cholesterol-free formula have increased cholesterol synthesis rates compared to breast-fed infants 36 . Interestingly, however, adult individuals who had been breast-fed as infant have lower total and pro-atherogenic LDL-cholesterol compared to previously formula-fed subjects 3 . This has led to the hypothesis that early life cholesterol supply can program cholesterol homeostasis in later life. In support of this notion, we recently reported indications that dietary cholesterol availability in early life of mice determines the set-point for cholesterol absorption efficiency at adult age 7 . The rather strictly regulated concentration of milk cholesterol found in this study could support the relevance of a stable cholesterol supply for  37 . Additionally, conversion of cholesterol to the neutral sterol coprostanol by the intestinal microbiota is delayed in breast-fed infants 38 , indicating inhibited growth of certain bacterial groups. Yet, a recent study by ourselves in (adult) LDLR-deficient mice does not support the concept that dietary cholesterol has a substantial impact on shaping the intestinal microbiota, since neither the composition nor the functionality of the intestinal microbiota were affected even after prolonged dietary cholesterol exposure 39 .
In conclusion, our results clearly demonstrate that milk cholesterol levels are resistant to maternal high cholesterol diet-and genetically-induced hypercholesterolaemia in mice. We speculate that the robust maintenance of stable milk cholesterol levels may serve relevant physiological functions in the offspring, such as programming of long-term health benefits. Further research however, is required to firmly establish such cause-effect relationships.

Materials and Methods
Animal studies. Female C57BL/6J (n = 10), Ldlr knockout 40 (n = 13) and Abcg8 knockout 20,41 (n = 9) mice were housed in temperature controlled-conditions with 12:12 light dark cycles and maintained on chow diet (RMH-B, ABDiets, Woerden, Netherlands) with listed specified ingredients: wheat, meat meal, yellow dent corn, whole oats, wheat middlings, alfalfa, soya oil, dried yeast, dicalcium phosphate, calcium carbonate, NaCl, dl-methionine, vitamins and trace elements. Breeding was initiated between 8-12 weeks of age. Due to accumulation of dietary xenosterols, Abcg8−/− mice are infertile, which is relieved upon ezetimibe treatment 42 . Therefore, in order to facilitate fertilization, Abcg8−/− females were pre-treated for 3 weeks with 0.005% ezetimibe provided via the food, which was removed from the diet once pregnancy was confirmed in accordance with Solca et al. 42 . The rest of the models were fed chow until E18, when half of the mice received 0.5% cholesterol diet. Lactation day 1 (L1) was considered the day at which pups were born. On L14 the dams were injected i.p. with 2.3 mL 99% 2 H 2 O (deuterium oxide) per 100 g BW, containing 0.9% NaCl. After 50 minutes, milk was collected for 10 minutes (details see below) directly followed by termination and harvesting of blood, liver, and mammary glands. All animal experiments were approved by the ethical committee for animal experimentation at the University of Groningen and performed in accordance with relevant guidelines and regulations.
Milk collection. At lactation day 14 43 , the dams were separated from the pups for 3 hours followed by i.p.
injection of 1 IU oxytocin (Synthocinon ® , Sigma-Tau Industrie Farmaceutiche Riunite, Rome, Italy). Milk samples were collected continuously for 10 minutes from the mammary gland of isoflurane-anesthetized mouse with the aid of a modified human electric breast pump (Calypso, Ardo Medical AG, Unterägeri, Switzerland). The samples were initially preserved at 4 °C during collection and further stored at −80 °C until use. Total plasma cholesterol and lipoprotein profiles. Total plasma cholesterol was measured enzymatically using a commercially available kit (Roche Diagnostics GmbH, Mannheim, Germany). Lipoprotein fractions of pooled plasma samples (n = 3-5) were separated via fast protein liquid chromatography gel filtration using a superose 6 column (GE Healthcare, Little Chalfont, UK) as published 46 . Samples were chromatographed at a flow rate of 0.5 ml/min, and lipoprotein fractions of 500 μl each were collected. Individual fractions were assayed for cholesterol concentrations using a commercial kit (Roche Diagnostics GmbH, Mannheim, Germany).

Determination of milk cholesterol.
Hepatic and mammary gland total cholesterol quantification. Liver and mammary gland tissues were homogenized using RNAse free-beads and the TissueLyser LT system (Qiagen GmbH, Hilden, Germany). Lipids were extracted according to Bligh & Dyer 44 . Cholesterol was de-esterified according to Ichihara et al. 47 . Free cholesterol underwent acetylation followed by quantification using gas chromatography (GC, Agilent 6890, Amstelveen, the Netherlands) 45 .
Organ-specific de novo cholesterol synthesis. Liver-and mammary gland-specific de novo cholesterol synthesis was quantified using the deuterium incorporation method 48 . Briefly, at L14 the dams were injected i.p. with deuterated water (2.3 mL/100 g BW, 99% 2 H 2 O, 0.9% NaCl) and terminated after 60 minutes by cardiac puncture 49 . We used a non-injected control mouse for determining the number of hydrogen atoms incorporated in a single newly synthesized cholesterol molecule as measured by GC-MS. Following lipid extraction and de-esterification, the abundance of deuterium-substituted hydrogen atoms was determined by isotope ratio mass spectrometry (IRMS). Synthesis rates were determined as previously published 50 . Statistical analysis. The significance of dietary influence within the different genotypes and the analysis of variance between genotypes in the same dietary condition was performed with Kruskal-Wallis followed by a multiple comparisons adjustment using Conover-Inman test. P-values below 0.05 were considered significant.
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.