Figure 5 | Scientific Reports

Figure 5

From: CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium

Figure 5

Genomic deletions and insertions in the targeting loci of PI3K genes. (A) The sequencing results of the targeting regions. Wild-type (WT) sequences of five PI3K genes (top) and sequences of 4 independent mutants are presented. The target sequence is in blue, and mutations are in red. Numbers in parentheses indicate the number of modified nucleotides. (B) Growth curve of the CRISPR mutants. Parental strain AX2 was used as a control.

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