Relevant substrates, intermediates, products, and enzyme kinetic parameters for (A) native Pdu microcompartment metabolism and (B) mevalonate biosynthesis. Predicted pathway flux for (C) native Pdu microcompartment metabolism and (D) mevalonate biosynthesis for native kinetics without organization; 100-fold improvement of k
for both pathway enzymes; 100-fold improvement in K
for both pathway enzymes; native kinetics with organization on a scaffold; and native kinetics with organization in a microcompartment organelle. The predictions here are based on an external substrate concentration of 50 mM 1,2-propanediol, as is typically used in experiments42. We use the same external substrate concentration (50 mM) in the mevalonate case. Experimental observations in (C) and (D) are calculated from S. enterica42 and from titer measurements for a scaffolded system from63.