Figure 8 | Scientific Reports

Figure 8

From: Mesenchymal stem cells and T cells in the formation of Tertiary Lymphoid Structures in Lupus Nephritis

Figure 8

Stimulated MSCs express higher levels of homeostatic cytokines and chemokines in co-culture with CD4+ T cells from the Healthy group. (a) MSCs (n = 7) were stimulated for 6 h with IL-1β and TNF-α (8 ng/ml) and analyzed for the mRNA expression of CCL19, IL-1β, TNF-α, ICAM1, CXCL13, LTβ, CCR5, CCR7, IDO, HLA-DRA, IL-6, IL-21 and IL-23 at day 0 and day 10. Results are given as FC mean ± SEM compared to nsMSCs at day 0. (b) nsMSCs and sMSCs in co-culture with CD4+ T cells at 1:100 ratio from the Healthy group (n = 3). Results are given as FC mean ± SEM compared to nsMSCs at day 10. (c) nsMSCs and sMSCs in co-culture with CD4+ T cells at 1:100 ratio from the LN group (n = 3). Results are given as FC mean ± SEM compared to nsMSCs at day 10. (One-way ANOVA with Post-hoc analysis Dunnett’s multiple comparisons test (compared within a group toward nsMSCs at given time point, marked with *). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.