Figure 6 | Scientific Reports

Figure 6

From: Mesenchymal stem cells and T cells in the formation of Tertiary Lymphoid Structures in Lupus Nephritis

Figure 6

CD4+ T cells proliferate more at high ratio in co-cultured with sMSCs. CD4+ T cells from (a,d) Healthy (n = 5), (b,e) SLE (n = 5) and (c,f) LN (n = 5) were co-cultured with sMSCs at (ac) 1:1 and (df) 1:100 ratio (MSCs:T cells) and proliferation was measured at day 5, 7 and 10. To investigate if the proliferation was contact dependent, CD4+ T cells from a Healthy donors (n = 2) were co-cultured with sMSCs and nsMSCs, or cultured alone at (g) 1:1 and (h) 1:100 ratio (MSCs: CD4+ T cells) in a Transwell system. Proliferation was measured at day 5, 7 and 10. CD4+ T cells from (i,j) Healthy (n = 3) were co-cultured with sHUV and nsHUV at 1:1 (i) and 1:100 ratio (j) and proliferation was measured at day 5, 7 and 10. Data are shown as optical density mean ± SEM. Two-way ANOVA and with post-hoc analyses Dunnett’s (compare different days within a group toward day zero, marked with #) and Tukey’s (compare stimulated and non-stimulated setups toward CD4+ T cells cultured alone at a given time point, marked with * and compare stimulated setup toward non-stimulated setup at a given time point, marked with+) multiple comparison tests. */#P < 0.05; **/##P < 0.01; ***/###P < 0.001; ****/####P < 0.0001. (*/#co-cultured with sMSCs or sHUV, */#co-cultured with nsMSCs or nsHUV).