Figure 5 | Scientific Reports

Figure 5

From: Mesenchymal stem cells and T cells in the formation of Tertiary Lymphoid Structures in Lupus Nephritis

Figure 5

Augmentation of proinflammatory cytokines and adhesion proteins in MSCs upon stimulation with TNF-α and IL-1β. MSCs (n = 3) were stimulated for 24 h with different concentrations of (a) TNF-α (b) IL-1β (c) IL-6 (d) IFN-α and (e) INF-γ. mRNA expressions are shown as FC mean ± SEM compared to control (Ctr). MSCs (n = 3) were stimulated with a combination of IL-1β and TNF-α (8 ng/ml) and mRNA expression of (f) CCL19, (g) IL-1β, (h) TNF-α, (i) ICAM1, and (j) VCAM1 were measured after 1–72 h. Results are shown as FC mean ± SEM compared to Ctr at the same time point. (k) The protein expression of MSCs were analyzed by Western blotting using antibodies against VCAM1, ICAM1, IL-1β, TNF-α and Actin. The quantitative ratio to actin was measured (k). (ae) Two-way ANOVA with Post-hoc analysis Dunnett’s multiple comparisons test within a group toward Ctr (*). (fj) Two-way ANOVA with Post-hoc analysis Sidak’s (compare between the groups Ctr and stimulated at given time point, marked with *) and Dunnett’s (compare within a group toward zero hour time point, marked with #) multiple comparisons tests. */#P < 0.05; **/##P < 0.01; ***/###P < 0.001; ****/####P < 0.0001.