Figure 6 | Scientific Reports

Figure 6

From: Optimized delivery of siRNA into 3D tumor spheroid cultures in situ

Figure 6

BSA can facilitate matrigel entry of siRNA, but not spheroid uptake. Representative confocal images of SW1463 spheroids showing localization of control-Cy3 siRNA at 2, 6 and 24 hours post transfection with siRNA formed in (A) 10% (B) 1% (C) 0.1% or (D) 0% BSA (no serum present) using RNAiMax. (E) Summary graph showing the mean internal spheroid fluorescence post transfection using siRNA formed with a dose-response of BSA. Data represents mean ± 1 s.d (n = 3).

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