Figure 4 | Scientific Reports

Figure 4

From: Optimized delivery of siRNA into 3D tumor spheroid cultures in situ

Figure 4

Matrigel and spheroid uptake of Opti-MEM-formed siRNA can be improved in the presence of 10% serum. (A) Representative confocal images of LS174T spheroids showing localization of control-Cy3 siRNA formed in 500 μl Opti-MEM using RNAiMax, but co-incubated in a well containing 1.5 mls DMEM containing 10% serum, at 2, 6 and 24 hours post transfection. (B) Representative confocal images of LS174T spheroids showing localization of control-Cy3 siRNA formed in 500 μl DMEM containing 10% serum using RNAiMax, but co-incubated in a well containing 1.5 mls Opti-MEM, at 2, 6 and 24 hours post transfection.

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