Figure 3 | Scientific Reports

Figure 3

From: Optimized delivery of siRNA into 3D tumor spheroid cultures in situ

Figure 3

Primary organoid uptake of siRNA is more efficient when complexes are formed with serum. Representative confocal images of VilCreER Apcfl/fl KrasG12D/+ primary mouse organoids showing localization of control-Cy3 siRNA 2, 6 and 24 hours post transfection with siRNA formed in (A) Opti-MEM or (B) 10% serum using RNAiMax. White scale bars indicate 150 μM. Representative confocal images of primary wild type mouse organoids showing localization of control-Cy3 siRNA 2, 6 and 24 hours post transfection with siRNA formed in (C) Opti-MEM or (D) 10% serum using RNAiMax. White scale bar indicates 80 μM except for 24 hours where it indicates 130 μM. White/black arrows indicate the location of the matrigel boundary.

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