Figure 2 | Scientific Reports

Figure 2

From: Optimized delivery of siRNA into 3D tumor spheroid cultures in situ

Figure 2

Failure of Opti-MEM prepared siRNA to penetrate matrigel or organoids is not cell line or transfection reagent specific. (A) Representative confocal images of LS174T spheroids showing localization of control-Cy3 siRNA formed in Opti-MEM using RNAiMax at 2, 6 and 24 hours post transfection. (B) Representative confocal images of LS174T spheroids showing localization of control-Cy3 siRNA formed in 10% serum using RNAiMax at 2, 6 and 24 hours post transfection. (C) Representative confocal images of LS174T spheroids showing localization of control-Cy3 siRNA formed in Opti-MEM using Lipofectamine 2000 at 2, 6 and 24 hours post transfection.

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