Analyses of complement markers in microglia. Mean fluorescence intensity (MFI) was used to compare the protein levels in microglia populations (CD11b+CD45low). (A,B) Comparison of C5aR MFI by surface staining on microglia. PLX5622 treatment after 50 cGy helium irradiation results in significantly reduced C5aR level compared to the 50 cGy-only group, **p < 0.01, One-way ANOVA with Sidak’s multiple comparisons test. (C,D) Comparison of CD11b MFI by surface staining on microglia. Statistics reveals no significant difference in CD11b (CR3A) levels across groups, but there is a trend of decreased MFI in the 50 cGy + PLX5622 group, p = 0.1096, unpaired student t-test. N = 3–4 each group.