Figure 3 | Scientific Reports

Figure 3

From: γδTCR regulates production of interleukin-27 by neutrophils and attenuates inflammatory arthritis

Figure 3

γδ T cell blockade increases IL-27 levels. (AF) Cytokine levels in serum of B10.RIII mice injected with GFP or IL-23 MC and treated with anti-γδ TCR or isotype mAb on day 11 (n = 4–5 per group). (G) qRT–PCR expression analysis of IL-27p28 mRNA expression in sorted macrophages, neutrophils and dendritic cells (DCs) isolated from TCRδ+/+ or TCRδ−/− mice after 5 hours of LPS stimulation. Data are representative of three independent experiments. All data are shown as mean ± SEM. ns, not significant (p > 0.05) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 as determined by using two-tailed Student’s t-test.