Aloperine treatment inhibited fibroblast differentiation. (A) Immunostaining of α-SMA expression in BLM-induced lung sections. Left panel: representative images for α-SMA expression. Right panel: quantitative mean fluorescence intensity of all mice studied. (B) Analysis of lung fibroblast differentiation after TGF-β stimulation. Up panel: representative western blot results for fibronectin, collagen I, vimentin, and α-SMA. Down panel: bar graphic figure for western blot results. (C) Analysis of lung fibroblast differentiation by RT-PCR following TGF-β. Statistical analysis was performed by Two-way ANOVA with Newman-Keuls post-hoc test or Mann-Whitney test. **P < 0.01; and ***P < 0.001.