Aloperine treatment suppresses fibroblast proliferation. (A) Results for Fsp1 expression in the lung sections. Left panel: representative immunostaining results of Fsp1. Right panel: bar graphic figure of Fsp1 for all mice examined. (B) Analysis of lung fibroblast proliferation after PDGF-BB stimulation. Left panel: representative EdU staining. Right panel: A bar graphic figure of EdU fluorescence intensity. (C) Analysis of cyclin D1expression. Left panel: representative western blot results for cyclin D1 expression. Right panel: graphic figures showing the western blot results. (D) The impact of aloperine on PDGF-BB stimulated PI3K/AKT/mTOR signaling in mouse lung fibroblasts. Left panel: representative western blot results for the levels of p-P85, p-AKT (Ser473 and Thr308), and p-mTOR. Right panel: graphic figures showing the western blot results with 3 replications. Statistical analysis was performed by Two-way ANOVA with Newman-Keuls post-hoc test or Mann-Whitney test. *p < 0.05; **p < 0.01; ***p < 0.001.