Analysis of ROS accumulation and apoptosis following BLM induction. (A) Analysis of ROS production. Left panel: representative results for detection of DCFH-DA fluorescence in lung sections. Right panel: quantitative mean fluorescence intensity of all mice studied. (B) Analysis of apoptosis. Left panel: Representative images for TUNEL assays of lung sections. Right panel: Quantitative analysis of TUNEL-positive cells. Images were taken under ×200 amplification. (C) Analysis of apoptosis-related proteins in lung homogenates. Left panel: representative western blot results for cleaved-caspase3, Bax, and Bcl-2 expression. Right panel: graphic figures showing the western blot results. Six to 7 mice were included in each study group. Statistical analysis was performed by one-way ANOVA with Newman-Keuls post-hoc test or Mann-Whitney test. **p < 0.01; ***p < 0.001.