Table 1 List of candidate reference genes (RGs). The amplicon size, PCR efficiency percentage, minimum and maximum threshold cycle (CT) values observed across all tested growth conditions, the standard deviation of CT values across all tested growth conditions (determined by BestKeeper), and the CT value of the no template control (NTC) are listed for each RG. See Supplementary Figure 1 for standard curves used to calculate PCR efficiency. See Supplementary Figure 2 for melting curve analysis. See Supplementary Table 1 for oligonucleotide sequences and melting and annealing temperatures.

From: Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630

Reference gene (RG) Gene number Gene annotation Amplicon size (bp) PCR efficiency Min CT Max CT CT Std Dev NTC CT
RG1 PD630_ RS22865 Pup-protein ligase 157 94% 23.44 25.23 0.40 N.d.
RG2 PD630_ RS20570 Hypothetical protein 87 94% 25.86 28.20 0.67 N.d.
RG3 PD630_ RS03840 23S rRNA 87 95% 8.74 10.16 0.33 N.d.
RG4 PD630_ RS15810 Polyribonucleotide nucleotidyltransferase 115 101% 22.93 27.41 1.37 N.d.
RG5 PD630_ RS25785 L,D-transpeptidase Mb0493 90 101% 24.56 25.96 0.37 N.d.
RG6 PD630_ RS13910 NAD(P)H dehydrogenase 82 100% 25.47 29.01 1.13 N.d.
RG7 PD630_ RS25530 ATP-dependent Clp protease ATP-binding subunit ClpX 94 92% 24.93 25.77 0.21 N.d.
RG8 PD630_ RS01395 16S rRNA 101 92% 10.48 11.74 0.29 N.d.
RG9 PD630_ RS37755 rRNA small subunit methyltransferase G 102 92% 28.32 30.33 0.46 N.d.
RG10 PD630_ RS27310 DNA polymerase IV 71 94% 28.34 30.61 0.61 N.d.