Figure 5 | Scientific Reports

Figure 5

From: Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630

Figure 5

Effect of reference gene choice on RT-qPCR normalization. (A) Box plots of averaged PD630_RS15810 expression data (CT) for all four growth conditions (HN, LN, TSB, and PHE). Each gray box represents the first through third quartiles, the solid black line represents the median, and the whiskers represent the minimum and maximum values. (BD) The normalized relative expression ratio of PD630_LPD05540 going from HN to either LN (B), TSB (C), or PHE (D). The expression data was normalized with a NF, including RG6, RG10, RG3/RG7, RG3/RG8, RG7/RG8, and RG3/RG7/RG8 using REST 2009. Error bars represent the 95% confidence interval (CI) as calculated by REST 2009. Stars indicate that a 95% CI range falls outside of the 95% CI range of the RG3/RG7/RG8 (and RG7/RG8) normalized ratio.