Figure 4 | Scientific Reports

Figure 4

From: Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630

Figure 4

Minimum number of reference genes. The pair-wise variation Vn/Vn + 1, where n represents the number of RGs used in the normalization factor (NF), was calculated by geNorm to determine the minimum number of RGs required for normalization. A geNorm V value below 0.15 signifies that no additional benefit is gained from increasing the number of reference genes from n to n + 1. The dashed line represents a V value of 0.15.

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