Figure 1 | Scientific Reports

Figure 1

From: Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630

Figure 1

Confirmation of specificity of primers used in RT-qPCR analysis. (A) Contrast enhanced image of electrophoresis gel confirming amplicon size and primer specificity using RT-qPCR amplification product. The sizes for nucleotide ladder are indicated to left of bands (50 to 200 bp). The size of each amplicon is denoted in Table 1. The original non-enhanced gel image, in addition to the corresponding gel image of no template controls (NTCs), can be found in Supplementary Figure 5. (B) Representative melt curves post RT-qPCR for RG1 and RG2 as well as the corresponding NTCs (see Supplementary Figure 2 for all melt curves).