Table 1 Steady-state kinetics of purified Luc proteins at pH 7.8.

From: A Firefly Luciferase Dual Color Bioluminescence Reporter Assay Using Two Substrates To Simultaneously Monitor Two Gene Expression Events

Enzyme kcat (s−1, x10−3) Km (µM) kcat/Km (mM−1s−1) LH2:BtLH2 (kcat/Km)
LH2 BtLH2 LH2 BtLH2 LH2 BtLH2
PpyWT 27 ± 3 2.8 ± 0.8 15 ± 2 6 ± 1 1.8 0.47 3.8
PLR1 12 ± 1 0.20 ± 0.03 37 ± 4 28 ± 3 0.32 0.007 45.7
PLG3 4.7 ± 0.3 2.3 ± 0.1 15 ± 3 5 ± 1 0.31 0.46 0.67