Comparison of the DART and Chroma-Glo™ Luciferase assay systems. Normalized spectral overlap measured with (a) DART and (b) Chroma-Glo™ using lysates from equal numbers of HEK293T cells transformed with (a) pCMV-PLG3 and pCMV-PLR1 assayed with DSM or (b) pCMV-CBG99 and pCMV-CBR assayed with Chroma-Glo™ reagent. Assays were performed using a Synergy™ 2 microplate luminometer equipped with the indicated filters. The relative emission from pCMV-PLR1 or pCMV-CBR detected through the 516 ± 10 nm filter (blue hatched bars) and the relative emission from pCMV-PLG3 or pCMV-CBG99 detected through the 635 ± 16 nm filter (red hatched bars) are shown. (c) BL emission from the same experiments detected through the indicated filters. The mean relative light units (RLU) ± STD for triplicate assays are depicted.