Figure 1 | Scientific Reports

Figure 1

From: A Firefly Luciferase Dual Color Bioluminescence Reporter Assay Using Two Substrates To Simultaneously Monitor Two Gene Expression Events

Figure 1

Bioluminescence emission and detectability of PLG3 and PLR1 assayed with DSM. (a) Photograph of in vitro BL reactions of 1 µg PLG3 and PLR1 with DSM. Chemical structures of the benzothiophene (BtLH2) and firefly (LH2) luciferins responsible for each emission color are shown. (b) Simultaneous detection of mixtures of lysates from equal numbers of HEK293T cells transfected with pCMV-PLG3 or pCMV-PLR1 using a Synergy™ 2 microplate reader equipped with narrow bandpass filters. The relative light units (RLU) measured through the 516 ± 10 nm filter (blue line) and 635 ± 16 nm filter (red line) represent the activity expressed from the pCMV-PLG3 and pCMV-PLR1 plasmids, respectively. (cd) BL emission spectra of lysates from equal numbers of HEK293T cells transformed with pCMV-PLG3 or pCMV-PLR1. The lysates were assayed (c) individually or (d) mixed together.

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