Expression of Cas mRNA and protein in trigeminal and nodose ganglia. (a–c) Whole-mount in situ hybridization for p130Cas, CasL and Sin in e11.5 embryos. White and black arrows mark the trigeminal ganglion and the nodose/petrosal complex, respectively. (d–i) In situ hybridization on transverse sections through the nodose (d–f) and trigeminal (g–i) of e11.5 embryos with probes against p130Cas (d,g), CasL (e,h) and Sin (f,i). (j–u) Expression profile of p130Cas protein (red) in transverse sections through the nodose (j–o) and trigeminal (p–u) ganglia at various developmental stages. Anti-neurofilament (2H3, green) was used to visualize axons and ToproIII (blue) was used to counterstain nuclei. Dotted lines delineate the ganglia. Scale bars: 500 μm for (a–c), 100 μm for (d–f), 200 μm for (g–i), 75 um for (j–o) and 150 um for (p–u).