p130Cas is phosphorylated in commissural axons and DRG central projections. (a–c) Expression profile of p130Cas protein (green) in transverse sections through the mouse spinal cord at e12.5. Anti-Neurofilament (2H3, red) was used to reveal axons. (d–f) Immunofluorescence for EGFP (green) and 2H3 (red) in e12.5 p130Cas EGFP-Bac spinal cords. ToproIII (blue) was used to counterstain nuclei. (g–i) Expression of a phosophorylated-p130Cas (PY-Cas, green) in e12.5 spinal cord and DRGs. (h) PY-Cas is present in the ventral funiculus and commissural axons. (i) p130Cas phosphorylation is mainly found in the dorsal spinal cord and is enriched in DRG axons and DREZ. White arrowheads: DREZ; Yellow arrowheads: DRG; White arrows: commissural axons; Yellow arrows: ventral funiculus; VR: ventral roots. Scale bar: 200 μm for (a–g) and 100 μm for (h–i).