Figure 5 | Scientific Reports

Figure 5

From: Identifying microRNA determinants of human myelopoiesis

Figure 5

Gene targets of miR-125b and miR-10a. Luciferase reporter assays performed in HeLa cells transfected with the WT or mutated 3′ UTR binding site from (a) MCL1, (b,c) FUT4 or (d) KLF4. The 3′ UTRs were cloned into a pSiCHECK2 vector and transfected with or without a specific inhibitor of mir-125b/ miR-10a or a non-specific control at 10 nM or 50 nM. The one predicted binding site in the MCL1 and KLF4 3′ UTRs were tested, while the two binding sites in FUT4 were each tested alone (Site 1 mutant and Site 2 mutant) and together (Double mutant). Each experiment was performed 3 times, each in triplicate. Graphs show mean ± SEM. Groups were tested by a two-way ANOVA with Bonferroni post tests. **p ≤ 0.01, * p ≤ 0.05, n.s. = not significant.