Figure 4 | Scientific Reports

Figure 4

From: Identifying microRNA determinants of human myelopoiesis

Figure 4

Identifying miRNA targets. Gene expression profiling was performed on progenitors, monocytes and granulocytes isolated from three human cord blood samples (1,2,3) using Affymetrix microarrays. The heatmaps show levels (log 2) of the top 2000 differentially expressed genes between (a) progenitors and monocytes and (b) progenitors and granulocytes. The identity of transcripts exhibiting higher expression in the mature cell types relative to progenitors was combined with target predictions for miR-125b (c) and miR-10a (d), both miRNAs with lower expression in the mature cells. The resulting 162 and 161 genes respectively (circled in red in c and d) that had inverse expression with the miRNA as well as being predicted targets were analysed further.