Decreased expression of miR-125b and miR-10a is associated with increased lineage commitment of myeloid progenitors. (a) qRT-PCR validation of miRNAs differentially expressed in TLDA experiments (n = 10). The small nucleolar RNA RNU24 was used for normalisation. Whiskers show 5th and 95th percentiles. miR-135a could not be amplified from most monocyte and neutrophil samples; the results have therefore been shown here as expression relative to RNU24 only. Differences were tested by ANOVA, Kruskal Wallis test with Dunn’s post test. ***p < 0.001, **p < 0.01, *p < 0.05, n.s. = not significant. (b) Expression of CD34, CD11b, CD14 and CD15 on MACS-purified cord blood CD34+ cells cultured for 6 days with SCF (100 ng/mL) and GCSF (10 ng/mL). (c) The same cytokines were used to in vitro differentiate FACS-purified CD34+ cells and their levels of miR-223, miR-125b and miR-10a assessed by q-RT-PCR over nine days (n = 3). Graphs show mean ± SEM.