Figure 1 | Scientific Reports

Figure 1

From: Identifying microRNA determinants of human myelopoiesis

Figure 1

Purification of myeloid populations. (a) FACS gating strategy for purifying the CD34+CD38+ myeloid progenitor populations. Firstly, mononuclear cells were gated on, then single cells, followed by lineage-negative cells. (b) Monocytes were identified as the CD11b+CD14+ cells within cord blood mononuclear cells. (c) Polymorphonuclear cells were isolated by dextran separation from red blood cells. Granulocytes were then sorted and were identified as the CD11bhighCD15high cells, following exclusion of CD235a+ red blood cells. (df) Myeloid progenitors were plated in semi-solid Methocult medium with the cytokines SCF, IL-3, GM-CSF and EPO. Colony types formed include colony forming unit-granulocyte/erythrocyte/monocyte/macrophage (d, left), granulocyte/macrophage (d, right), granulocyte (e, left), macrophage (e, right), erythrocyte (f, left) and blast forming unit-erythrocyte (f, right). Note: Images df are on different scales to emphasize morphological features.