Figure 2: Scientific Reports

Figure 2

From: Abundant fish protein inhibits α-synuclein amyloid formation

Figure 2

Amyloid formation of mixtures of αS and apo/holo PV. (a) ThT fluorescence as a function of time for 70 µM αS (with and without Ca) and αS + 280 µM holo-PV. (b) ThT fluorescence as a function of time for 70 µM αS, 280 µM apo-PV, and mixture of αS with apo-PV. The expected signal for the αS and apo-PV mixture, if both proteins aggregated independently, are shown as a dashed curve. (c) Amyloid fiber height analysis (based on 15 different fibers in each case) in the αS/apo-PV mixture, αS alone and apo-PV alone samples. (d) AFM image of end point in ThT experiments for αS/apo-PV mixture (more views in Figure S2B).