Temporal changes in the co-cultured tumor and bone spheroids. Of note, MC = MC3T3 osteoblast-like cells, and A5 = MLO-A5 osteocyte-like cells. (A & B) Shape changes by spheroid fusion of TMD or BMD tumor spheroids with MC3T3, MLO-A5, or MLO-Y4 bone spheroids. (C & D) Changes in the cross-sectional area of TMD and BMD spheroids, respectively. (E) Changes in the cross-sectional area of TMD spheroids (green). Control = no treatment, A5 fusion = fusion with MLO-A5 spheroid (red), and A5 media = culture in conditioned medium isolated from MLO-A5 osteocyte-like cells. (F) Shrinkage of E0771 spheroids by MLO-Y4 conditioned medium.