Figure 8 | Scientific Reports

Figure 8

From: Protein-bound NAD(P)H Lifetime is Sensitive to Multiple Fates of Glucose Carbon

Figure 8

Effects of FX11 and DCA on the fluorescence lifetime of protein-bound NAD(P)H in HPDE6 cells. (a) Representative images of HPDE6 cells after 48 hours of 10 μM FX11 treatment vs. vehicle, color-coded for NAD(P)H \({\tau }_{2}\). Scale bar = 50 μm. (b) Mean and standard deviations of NAD(P)H \({\tau }_{2}\) in HPDE6 cells after 48 hours of 10 μM FX11 treatment vs. vehicle. *p < 0.05 vs. control. n = 4 experiments. (c) Representative images of HPDE6 cells after 48 hours of 50 mM DCA treatment vs. vehicle color-coded for NAD(P)H \({\tau }_{2}\). (d) Mean and standard deviations of NAD(P)H \({\tau }_{2}\) in HPDE6 cells after 48 hours of 50 mM DCA treatment vs. vehicle. n = 4 experiments.