Figure 2 | Scientific Reports

Figure 2

From: Protein-bound NAD(P)H Lifetime is Sensitive to Multiple Fates of Glucose Carbon

Figure 2

FLIM of NADH quantifies a mixture of enzymes in solution. The relationship between the relative concentration of LDH in a solution of NADH, LDH, and MDH, and the measured \({\alpha }_{NADH-LDH}\) value (Eq. 4). Error bars represent the standard deviation across triplicate experiments, and the dotted line represents the 95% confidence interval for the regression line. Error bars on one of the points are too small to display (SD =  ± 0.004). The linear regression is forced through 0,0, and the slope of the line is given as m. [LDH], concentration of LDH in the solution; [LDH + MDH], concentration of LDH plus MDH in the solution. \({\alpha }_{NADH-LDH}\) and \({\alpha }_{NADH-MDH}\) from Eq. 4.