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Figure 1

From: Protein-bound NAD(P)H Lifetime is Sensitive to Multiple Fates of Glucose Carbon

Figure 1

FLIM of NADH bound to metabolic enzymes in solution. (a) Mean and standard deviations of the fluorescence lifetime of 50 μM NADH and 50 μM NADPH from 3–5 experiments. ns = p > 0.05. (b,c) Mean and standard deviations of the fraction of protein-bound NADH, or \({\alpha }_{2}\), measured in mixtures of 50 μM NADH with varied concentrations of (b) MDH or (c) LDH across triplicate experiments. *p < 0.05. (d) Mean and standard deviations of NADPH \({\alpha }_{2}\) measured in mixtures of 50 μM NADPH with varied concentrations of G6PDH across triplicate experiments. (e–g) Mean and standard deviations of the protein-bound lifetime of NAD(P)H, or \({\tau }_{2}\), measured in the same solutions of NADH with MDH (e), NADH with LDH (f), and NADPH with G6PDH (g) across triplicate experiments.

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