Table 1 Michealis-Menten constants (K M ) and maximal uptake rate (V max ) values for glucose transport after pre-growth on maltose.

From: Low affinity uniporter carrier proteins can increase net substrate uptake rate by reducing efflux

Strain KM (mM) V max
Kasahara et al.19 Our strains Kasahara et al.19 pmol (5 s)−1(107 cells)−1 (ratio vs WT) Our strains μ mol min−1(mg protein)−1 (ratio vs WT)
Low affinity T213N 34 ± 4 43 ± 11 960 ± 50 (1.7) 1.67 ± 0.19 (4.3)
High affinity T213T (WT) 0.72 ± 0.07 0.67 ± 0.05 560 ± 30 (1) 0.39 ± 0.08 (1)