Figure 4 | Scientific Reports

Figure 4

From: Low affinity uniporter carrier proteins can increase net substrate uptake rate by reducing efflux

Figure 4

Maximal uptake rate (V max ) values and steady-state fluxes for different pre-growth conditions. (A) V max values were measured in a 5 s zero-trans uptake assay with 200 mM glucose as substrate. Pre-growth was done on 2% of the carbon source indicated. Prior to the experiment cells were washed in medium without carbon source and incubated for 3′ at 30 °C without a carbon source. Experiments were done in triplicate and shown are the averages with standard deviation. (B) Glucose and ethanol concentrations were measured during two hours at 30 °C following a glucose pulse. Protein concentration remained constant throughout the experiment. From this data, specific exchange fluxes were calculated. Shown is the average and standard deviation of two incubations per pre-growth condition.

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