Figure 3 | Scientific Reports

Figure 3

From: Low affinity uniporter carrier proteins can increase net substrate uptake rate by reducing efflux

Figure 3

Experimental data suggest a rate-affinity trade-off. Both panels show experimentally established affinities and rates of HXT mutants. (A) HXT1-HXT7 chimeras. Data taken from Elbing et al.10 (Converted from per mg of protein to per 107 cells using 5 pg protein/cell, BioNumber ID:110550) (B) Different HXT2 and HXT7 mutants and chimeras constructed and characterised in the Kasahara lab. References are in the figure legend. The data suggest the existence of a k cat − 1/K M Pareto-front (gray dashed line), provided the expression levels of all constructs is comparable. The wild-type transporters are located on this front.