Figure 9 | Scientific Reports

Figure 9

From: Inhibitory effect of surface pre-reacted glass-ionomer (S-PRG) eluate against adhesion and colonization by Streptococcus mutans

Figure 9

The in vitro properties of Streptococcus sobrinus B13 and Streptococcus gordonii ATCC10558 in the presence of various concentrations of S-PRG eluate. Bacterial growth using S. sobrinus before reaching the logarithmic phase (adjusted to 1 × 105 CFU/ml) (A) and in the post-logarithmic phase (adjusted to 1 × 109 CFU/ml) (B) following 18 h incubation, which was determined by recovered bacterial numbers on MSB plates. Bacterial growth using S. gordonii reaching the logarithmic phase (adjusted to 1 × 105 CFU/ml) (C) and in the post-logarithmic phase (adjusted to 1 × 109 CFU/ml) (D) with 18 h incubations, which was determined by recovered bacterial numbers on MSB plates. Sucrose-dependent adhesion rates using bacterial suspensions of S. sobrinus B13 adjusted before reaching the logarithmic phase (adjusted to 1 × 107 CFU/ml) (E) and in the post-logarithmic phase (adjusted to 1 × 109 CFU/ml) (F), respectively. Quantitation of biofilm formation using bacterial suspensions of S. sobrinus B13 adjusted to 1 × 107 CFU/ml (G) and 1 × 109 CFU/ml (H), respectively. Significant differences were determined using ANOVA with Bonferroni correction. *P < 0.05, **P < 0.01, and ***P < 0.001 versus no S-PRG eluate.