The fecal metabolome of 3xtg mice is altered with respect to control mice. Relative fold changes for in test groups (HFD, and 3xtg mice under ND or HFD) versus control ND mice, derived from NMR integrals of metabolic regions with higher VIP scores in the PLS-DA analysis in colon and caecum faecal extracts. Bars represent fold-change in metabolite content in each comparison (increased content: positive bars; decreased content: negative bars). ND, ND-fed control mice; 3xtg, ND-fed 3xtg mice; HFD, HFD-fed control mice; 3xtgHFD, HFD-fed 3xtg mice. *p < 0.05; **p < 0.01; ***p < 0.001.